Key result
Exposure of HUVECs to 2 Gy radiotherapy significantly increased miRNA-221 and miRNA-222 and reduced miRNA-155 at 2 hours, while overexpressing miRNA-146a and miRNA-155 at 24 hours.
Why the study?
Radiotherapy can lead to cardiovascular disease, and evidence suggests radiation modulates miRNA levels, but the acute response of specific miRNAs, inflammation, and endothelial dysfunction in endothelial cells required assessment.
Does 2 Gy radiotherapy modulate the expression of CVD-related miRNAs and inflammatory markers in human umbilical vein endothelial cells?
Does 2 Gy radiotherapy modulate the expression of CVD-related miRNAs and inflammatory markers in human umbilical vein endothelial cells?
A 2 Gy dose of radiation acutely modulates the expression of specific cardiovascular disease-related miRNAs in endothelial cells, providing a potential mechanistic link to radiation-induced cardiovascular disease.
May signal endothelial vulnerability to radiotherapy; leaves open whether miRNA modulation drives clinical CVD.
BACKGROUND/AIM: Radiotherapy (RT) can lead to cardiovascular disease (CVD). Evidence suggests that radiation modulates miRNA levels. Our purpose was to assess the acute response to radiation-induced modulation of the expression of miRNA-146a, miRNA-155, miRNA-221, and miRNA-222, inflammatory response and endothelial dysfunction on endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVECs) were exposed to 2 Gy RT, and intracellular levels of selected miRNAs were measured by real-time polymerase chain reaction at 2 and 24 h. Cytokine and adhesion molecule release were also assessed. RESULTS: Results showed that 2 Gy significantly increased the expression of miRNA-221 and miRNA-222, and reduced the level of miRNA-155 after 2 h; whereas miRNA-146a and miRNA-155 were significantly overexpressed and miRNA-222 was significantly down-regulated at 24 h. Interleukin-8 and soluble vascular cell adhesion molecule 1 levels were not affected by the studied RT. CONCLUSION: RT at 2 Gy modulated expression of selected miRNAs by endothelial cells after 2 and 24 h, which might be related to CVD development in patients who receive RT.
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Esplugas et al. (2019) studied this question. Radiotherapy (RT) was evaluated on Expression of miRNA-146a, miRNA-155, miRNA-221, and miRNA-222, inflammatory response and endothelial dysfunction. Exposure of HUVECs to 2 Gy radiotherapy significantly increased miRNA-221 and miRNA-222 and reduced miRNA-155 at 2 hours, while overexpressing miRNA-146a and miRNA-155 at 24 hours.
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