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Elucidate the biosynthetic pathway and molecular assembly of mollisin using carbon-13 isotopic labeling.

Utilization of 13 C– 13 C coupling in structural and biosynthetic studies; the Fourier transform 13 C nuclear magnetic resonance spectrum of mollisin

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Elucidate the biosynthetic pathway and molecular assembly of mollisin using carbon-13 isotopic labeling.
Incorporated doubly labeled [1,2-13C]acetate precursors into mollisin.
Analyzed 13C–13C spin coupling patterns using Fourier transform 13C nuclear magnetic resonance (FT 13C-NMR) spectroscopy.
Resolved distinct 13C–13C coupling networks corresponding to intact precursor units across the mollisin framework.
Clarified the sequence of carbon bond formations governing the biosynthetic assembly of mollisin.

Spectroscopic analysis uncovers the biosynthetic pathway of mollisin using acetate labeling, highlighting the utility of carbon-13 coupling.

The biosynthetic pathway to mollisin has been clarified by the use of [1,2-13C]acetate and FT 13C-n.m.r.

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Seto et al. (1973) studied this question.

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Spectroscopic analysis uncovers the biosynthetic pathway of mollisin using acetate labeling, highlighting the utility of carbon-13 coupling.

The biosynthetic pathway to mollisin has been clarified by the use of [1,2-13C]acetate and FT 13C-n.m.r.