Key result
An ultrahigh-performance liquid chromatography-tandem mass spectrometry method successfully measured 74 oxylipins in plasma samples from 302 myocardial infarction patients and 99 healthy individuals.
Why the study?
Oxylipins play a role in inflammation accompanying myocardial infarction, and analyzing a wide panel may help comprehensively elucidate signaling mediator profiles and myocardial infarction.
Does the profile of oxylipins measured by UHPLC-MS/MS differ between myocardial infarction patients and healthy individuals?
Observational (n=401)
Does the profile of oxylipins measured by UHPLC-MS/MS differ between myocardial infarction patients and healthy individuals?
A newly developed UHPLC-MS/MS method allows for rapid and accurate profiling of 74 oxylipins, identifying potential biomarkers for myocardial infarction.
New UHPLC-MS/MS method enables oxylipin profiling in MI; leaves open biomarker utility pending validation.
RATIONALE: Oxylipins play an important role in inflammatory processes, accompanying the occurrence of myocardial infarction. Analyzing a wide panel of oxylipins derived from more polyunsaturated fatty acids may provide valuable information to elucidate the relationships between the signaling mediator profile and myocardial infarction comprehensively. METHODS: An ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneously measuring 74 oxylipins in 50-μL human samples with a 10-min chromatographic run and easy operation has been developed and validated. RESULTS: Accuracy and precision were below 15% of the relative errors in 99% of quality control. Recoveries and matrix effect were considered acceptable. Potential effects of different collecting tubes were also assessed. We successfully utilized our approach to measure plasma samples obtained from 99 healthy individuals and 302 myocardial infarction patients. CONCLUSIONS: Profiles of oxylipins discovered potential biomarkers and clarified the pathological characteristics of oxylipin metabolism in myocardial infarction. Our approach was rapid, accurate, and precise, with high throughput, low sample volume, and easy operation, suitable for large-scale studies.
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Tan et al. (2025) conducted an observational in Myocardial infarction (n=401). Myocardial infarction vs. Healthy individuals was evaluated on Oxylipin profiles. An ultrahigh-performance liquid chromatography-tandem mass spectrometry method successfully measured 74 oxylipins in plasma samples from 302 myocardial infarction patients and 99 healthy individuals.
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