Key result
Intravenous infusion of human apoA-I prevented deep vein thrombosis in wild-type mice, an effect mediated by the extrahepatic HDL receptor SR-BI and endothelial nitric oxide synthase.
Why the study?
Does intravenous infusion of human apoA-I prevent deep vein thrombosis in a murine model?
Does intravenous infusion of human apoA-I prevent deep vein thrombosis in a murine model?
The apoA-I (HDL)-SR-BI-eNOS axis protects against deep vein thrombosis in mice, suggesting a potential new target for DVT prophylaxis and treatment.
Hypothesis-generating for apoA-I in DVT prevention; requires human validation before clinical consideration.
OBJECTIVE: Deep vein thrombosis (DVT) and pulmonary embolism are frequent causes of morbidity and mortality. The goal of our study was to determine whether plasma high-density lipoprotein (HDL), which inversely correlates with the risk of cardiovascular events, affects DVT. METHODS AND RESULTS: Using a murine DVT model of inferior vena cava stenosis, we demonstrated that deficiency of the HDL receptor, scavenger receptor class B type I (SR-BI), promotes venous thrombosis. As SR-BI(-/-) mice have increased plasma cholesterol levels and abnormal HDL particles, we tested SR-BI(-/-) mice with an SR-BI liver transgene that normalizes both parameters. These mice also exhibited increased susceptibility to DVT, indicating a protective role of extrahepatic SR-BI. Mice lacking the major HDL apolipoprotein apoA-I or endothelial nitric oxide synthase (eNOS) (a downstream target of endothelial SR-BI signaling) also had a prothrombotic phenotype. Intravenous infusion of human apoA-I, an HDL component and SR-BI ligend, prevented DVT in wild-type but not SR-BI(-/-) or eNOS(-/-) mice, suggesting that its effect is mediated by SR-BI and eNOS. Intravenous apoA-I infusion abolished histamine-induced platelet-endothelial interactions, which are important for DVT initiation. CONCLUSIONS: An apoA-I (HDL)-SR-BI-eNOS axis is highly protective in DVT and may provide new targets for prophylaxis and treatment of venous thrombosis.
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Brill et al. (2012) studied Deep vein thrombosis. Intravenous infusion of human apoA-I vs. Wild-type mice / SR-BI(-/-) mice / eNOS(-/-) mice was evaluated on Venous thrombosis (DVT). Intravenous infusion of human apoA-I prevented deep vein thrombosis in wild-type mice, an effect mediated by the extrahepatic HDL receptor SR-BI and endothelial nitric oxide synthase.
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