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Stem explants of the non-legume Parasponia andersonii produced callus which regenerated shoots when cultured on a high-cytokinin, Murashige and Skoog (1962) -based medium. After transfer to a high auxin containing medium followed by P medium (Bender and Rolfe, 1985), the shoots produced roots which developed effective nodules when inoculated with Bradyrhizobium.
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Davey et al. (1993) studied this question.
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