Key result
A method for isolating, expanding, and characterizing cardiac mesenchymal stromal cells from endomyocardial biopsies of arrhythmogenic cardiomyopathy patients is described.
The described method enables the isolation and study of primary cardiac mesenchymal stromal cells from ACM patients, providing a valuable in vitro model for investigating the disease's adipogenic mechanisms.
Facilitates patient-derived models for arrhythmogenic cardiomyopathy research; leaves open clinical translation pending validation.
A normal adult heart is composed of several different cell types, among which cardiac mesenchymal stromal cells represent an abundant population. The isolation of these cells offers the possibility of studying their involvement in cardiac diseases, and, in addition, provides a useful primary cell model to investigate biological mechanisms. Here, the method for the isolation of C-MSC from arrhythmogenic cardiomyopathy patients' bioptic samples is described. The endomyocardial biopsy sampling is guided in the right ventricular areas adjacent to the scar visualized by electro-anatomical mapping. The digestion of the biopsies in collagenase and their plating on a plastic dish in culture medium to allow C-MSC growth is described. The isolated cells can be expanded in culture for several passages. To confirm their mesenchymal phenotype, the description of immuno-phenotypical characterization is provided. C-MSC are able to differentiate into several cell types like adipocytes, chondrocytes, and osteoblasts: in the context of ACM, characterized by adipocyte deposits in patients' hearts, the protocols for the adipogenic differentiation of C-MSC and the characterization of lipid droplet accumulation are described.
No takes yet. Share an insight, caveat, or question.
Pilato et al. (2018) studied Arrhythmogenic Cardiomyopathy. Isolation and characterization of cardiac mesenchymal stromal cells was evaluated on Method description for isolation, expansion, and adipogenic differentiation of C-MSC. A method for isolating, expanding, and characterizing cardiac mesenchymal stromal cells from endomyocardial biopsies of arrhythmogenic cardiomyopathy patients is described.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: