Key result
MiR-195 inhibited proliferation and induced apoptosis of vascular smooth muscle cells, promoting media remodeling by targeting Smad3 in abdominal aortic aneurysm progression.
Why the study?
Does miR-195 regulate proliferation and apoptosis of vascular smooth muscle cells by targeting Smad3 in the context of abdominal aortic aneurysms?
Does miR-195 regulate proliferation and apoptosis of vascular smooth muscle cells by targeting Smad3 in the context of abdominal aortic aneurysms?
MiR-195 promotes media remodeling in abdominal aortic aneurysms by targeting Smad3, suggesting a potential novel therapeutic target.
Suggests miR-195/Smad3 axis as AAA target; hypothesis-generating and leaves open human validation.
INTRODUCTION: MiR-195 has been implicated in the development of abdominal aortic aneurysms (AAA). However, the underlying mechanisms have not been fully addressed. AIM: To explore the roles of miR-195 in regulating the proliferation and apoptosis of vascular smooth muscle cells (VSMCs), as well as its molecular basis in vitro. METHODS: qRT-PCR was used to determine the expression levels of miR-195 and Smad3 in aortic media specimens or VSMCs. Western blot was performed to detect the protein levels of Smad3, osteopontin (OPN), and collagen III in aortic media specimens and VSMCs. Luciferase reporter assay was applied to confirm the target of miR-195 in VSMCs. Proliferation and apoptosis of VSMCs were measured by MTT and flow cytometry, respectively. RESULTS: In comparison with the normal controls, the levels of miR-195, OPN, and collagen III were significantly increased in AAA tissue. Smad3 was validated to be a direct target of miR-195. miR-195 inhibited proliferation and induced apoptosis of VSMCs, which was abated by Smad3 overexpression. Expression of OPN and collagen III was improved in VSMCs after transfection with miR-195 mimics, while up-regulation of Smad3 reversed this effect. CONCLUSION: MiR-195 promotes media remodeling by targeting Smad3 in AAA progression. This study suggests that miR-195 contributes to the pathogenesis of AAA and reveals a new targeted therapy strategy for AAA patients.
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Liang et al. (2017) studied Abdominal aortic aneurysm. miR-195 vs. Normal controls / Smad3 overexpression was evaluated on Proliferation and apoptosis of vascular smooth muscle cells. MiR-195 inhibited proliferation and induced apoptosis of vascular smooth muscle cells, promoting media remodeling by targeting Smad3 in abdominal aortic aneurysm progression.
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