Identification of sources of resistance to wheat stem rust using molecular markers

Stem rust is one of the most harmful wheat diseases. The Ug99 race of the causative agent of the disease ( Puccinia graminis Perf. f. sp. tritici ), which is virulent to most varieties of wheat, has a pathotype TTKS composition. According to Food and Agriculture Organization (FAO) forecasts, this disease may spread eastward of Iran into many countries of Central Asia, including Kazakhstan. The Sr2, Sr22, Sr24 / Lr24 , and Sr46 genes provide protection from the Ug99 race. Screening of samples for the presence of efficient genes of the stem rust resistance was conducted for a promising wheat breeding. Using molecular markers, the Sr2 gene was identified in 12 samples; the Sr24 / Lr24 gene, in 7 samples; the Sr46 gene, in 1 sample; and the Sr22 gene, in 6 lines. The results obtained are used in Kazakhstan for developing varieties of wheat that are resistant to stem rust (using MAS selection).