Electrophoresis of Lymphoid Cells

The electrophoretic mobility of small lymphoid cells in the thymus, lymph nodes, and spleen was measured. The distribution patterns were organ specific. The majority of the thymic cells were slow with a small ‘tail’ of faster cells. Both lymph node and spleen cells showed a fast and a slow cell population with a difference in mean electrophoretic mobility of about 40%. The percentage of cells to the fast and slow groups was similar to the percentage of T‐ and B‐cells in these organs It is therefore suggested that T‐and B‐cells may be characterized by their different electrophoretic mobilities and also that there may be a change in the net surface charge during maturation from thymocyte to T‐lymphocyte.