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An isocratic reversed-phase liquid chromatographic method for the determination of eight cephalosporins in human plasma using UV detection at 254 nm is described. Plasma proteins were precipitated using acetonitrile prior to injection of a 10 μl aliquot onto an octadecylsilane column. The mobile phases consisted of 6–11% acetonitrile in sodium dihydrogen phosphate (0.01M). The minimum detectable limit for each drug was less than 1 γg/ml of plasma. Possible interference from other drugs which might be administered concurrently is discussed. The reproducibility and precision of the method for cephalosporin assay are shown from the analysis of plasma containing 5–500 γg/ml of plasma. The chromatographic behavior of the eight cephalosporins was examined by varying mobile phase conditions.
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Nygard et al. (1984) studied this question.
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