DNA Polyhedra with T-Linkage

This paper reports a strategy for DNA self-assembly. Cross-over-based DNA nanomotifs are held together by T-junctions instead of commonly used sticky-end cohesion. We have demonstrated this strategy by assembling a DNA tetrahedron, an octahedron, and an icosahedron. The resulting DNA polyhedra contain out-pointing, short DNA hairpin spikes. These hairpins are well-structured relative to the polyhedra core and provide potential locations for introduction of functional chemicals such as proteins and gold nanoparticles. The T-linked DNA polyhedra have been characterized by polyacrylamide gel electrophoresis, atomic force microscopy, and dynamic light scattering.