Tandem Acyl Carrier Proteins in the Curacin Biosynthetic Pathway Promote Consecutive Multienzyme Reactions with a Synergistic Effect

Synergistic gathering: Biochemical studies reveal a highly efficient strategy in biosynthesis to promote consecutive multienzyme reactions. In the curacin A pathway, catalytic efficiency of a multi-step modification process and one-step translocation is significantly enhanced by tandem organization and dimerization of the ACP3 tridomain with an unexpected synergistic effect (see scheme).