The biological activity of bacterial vaccine of Pseudomonas putida2 and Pseudomonas fluorescens3 isolates to protect sesame crop (Sesamum indicum) from Fusarium fungi under field conditions

This research investigated the biological effects of Pseudomonas putida2 and Pseudomonas fluorescens3 as biocides to inhibit Fusarium fungi growth and as biofertilizers to improve growth characters of sesame crop grown in contaminated soil with Fusarium under field conditions compared with Dithen. Results showed mixture of vaccine Pseudomonas putida2 + Pseudomonas fluorescence3 together was more effect on Fusarium growth and increased growth characters much higher than each isolate alone. Both isolates scored significant improving in morphological, physiological and productivity characters for sesame compared with control and Dithen treatments. But mixture of P. putida2 + P. fluorescens3 treatment together (Fusant) as a biocide and biofertilizers gave higher significant results in increasing chlorophyll content, percentage of N, P, K in total dry weight of shoot, branch no./plant, height of plant, leaf area per plant, leaf no./plant, pods no./plant, grains no./pod, total weight of 1000 grains, total yield of grains/plot, and percentage of oil in sesame grains. The values were 3.21 mg/gm, 4.18%, 0.44%, 3.87%, 45.8 branch/plant, 151.7 cm/plant, 59.7 cm 2 /plant, 428.3 leaf/plant, 146.7 pod/plant, 69.1 grain/pod, 2.92 gm/1000 grain, 982.3 gm (grains)/plot and 56.2 % oil in sesame grains respectively. While control treatment scored: 0.85 mg/gm, 1.77%, 0.11%, 1.43%, 14.6 branch/plant, 53.3 cm/plant, 25.5 cm 2 /plant, 162.7 leaf/plant, 44.0 pod/plant, 31.3 grain/pod, 0.94 gm/1000 grain, 112.4 gm (grains)/plot and 26.6 % oil in sesame grains respectively.