RAPD (randomly amplified polymorphic DNA) analysis was developed for rapid identification of the spoilage bacterium, Alicyclobacillus acidoterrestris. Three primers, Ba-10, F-61, and F-64, allowed adequate discrimination between strains of A. acidoterrestris and related bacteria in RAPD analyses. As compared with the conventional method and a RAPD assay against the isolates from various environmental and food samples, the results obtained from the RAPD pattern were identical to the identification by the conventional method. We suggest that the RAPD analysis is a rapid and reliable technique to distinguish A. acidoterrestris from other related bacteria.
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Yamazaki et al. (1997) studied this question.
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