Long Noncoding RNA AF131217.1 Regulated Coronary Slow Flow-Induced Inflammation Affecting Coronary Slow Flow via KLF4

Figures

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  Fig. 1. Long noncoding ribonucleic acid (lncRNA) AF131217.1 expression in the coronary slow flow (CSF) model. Heat map and volcano figure of gene chip (A and B), quantitative real-time polymerase chain reaction of lncRNA AF131217.1 expression (C) in the CSF model. The mean thrombolysis in myocardial infarction frame count (TFC) was positively correlated with lncRNA AF131217.1 levels (D) and high-sensitivity C-reactive protein (hsCRP) levels (E). Control, normal volunteer group; hsCRP, hsCRP patients group. ##P<0.01 compared with normal volunteer group.

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  Fig. 2. Long noncoding ribonucleic acid (lncRNA) AF131217.1 regulated inflammation in the in vitro model. LncRNA AF131217 expression (A), tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-1β, and IL-18 (B, C, D, and E) in overexpression of lncRNA AF131217 group; lncRNA AF131217 expression (F), TNF-α, IL-6, IL-1β, and IL-18 (G, H, I, and J) in downregulation of lncRNA AF131217 group. LPS+Negative, LPS-induced in vitro model by negative mimics group; LPS+AF131217.1, LPS-induced in vitro model by AF131217.1 group. ##P<0.01 compared with LPS+Negative group. LPS=lipopolysaccharide.

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  Fig. 3. Micro ribonucleic acid (miR)-128-3p is a target spot of long noncoding ribonucleic acid (lncRNA) AF131217.1 on the inflammation in vitro model. MiR-128-3p expression had a negative correlation with lncRNA AF131217.1 levels in the coronary slow flow model (A). The luciferase reporter plasmid containing wild type (WT) or mutant (Mut) AF131217.1 was cotransfected with miR-128-3p (B and C), overexpression of lncRNA AF131217.1 suppressed miR-128-3p expression (D), downregulation of lncRNA AF131217.1 induced miR-128-3p expression in lipopolysaccharide (LPS)-induced in vitro model (E). LPS+Negative, LPS-induced in vitro model by negative mimics group; LPS+AF131217, LPSinduced in vitro model by AF131217.1 group. ##P<0.01 compared with LPS+Negative group.

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  Fig. 4. Micro ribonucleic acid (MiR)-128-3p and Kruppel-like factor (KLF) 4 expressions in the coronary slow flow (CSF) model (A and B), miR- 128-3p expression had a negative correlation with KLF4 levels in the rat model (C). Control, normal volunteer group; CSF, CSF patients group. ##P<0.01 compared with normal volunteer group. mRNA=messenger ribonucleic acid

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  Fig. 5. Micro ribonucleic acid (MiR)-128-3p regulated inflammation in the in vitro model. MiR-128-3p expression (A), tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-1β, and IL-18 (B, C, D, and E) in overexpression of miR-128-3p group; miR-128-3p expression (F), TNF-α, IL-6, IL-1β, and IL-18 (G, H, I, and J) in downregulation of miR-128-3p group. LPS+Negative, LPS-induced in vitro model by negative mimics group; LPS+miR-128-3p, LPS-induced in vitro model by miR-128-3p group. ##P<0.01 compared with LPS+Negative group. LPS=lipopolysaccharide

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  Fig. 6. Kruppel-like factor (KLF) 4 is a target spot of micro ribonucleic acid (MiR)-128-3p on the inflammation in vitro model. The luciferase reporter plasmid containing wild type (WT) or mutant (Mut) KLF4 was cotransfected with miR-128-3p (A and B), KLF4, RhoF, and nuclear factor kappa B (NF-κB) protein expressions (C, D, E, and I) by overexpression of miR-128-3p; KLF4, RhoF, and NF-κB protein expressions (F, G, H, and J) by downregulation of miR-128-3p. LPS+Negative, LPS-induced in vitro model by negative mimics group; LPS+miR-128-3p, LPS-induced in vitro model by miR-128-3p group; LPS+simiR-128-3p, LPS-induced in vitro model by simiR-128-3p group. ##P<0.01 compared with LPS+Negative group. GAPDH=glyceraldehyde-3-phosphate dehydrogenase; LPS=lipopolysaccharide

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  Fig. 7. Long noncoding ribonucleic acid AF131217.1 regulated inflammation in the in vitro model via Kruppel-like factors (KLF) 4 by micro ribonucleic acid (miR)-128-3p. MiR-128-3p expression (A), tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-1β, IL-18, KLF4, RhoF, and nuclear factor kappa B (NF-κB) protein expressions (B, C, D, E, F, G, H, and I). LPS+Negative, LPS-induced in vitro model by negative mimics group; LPS+AF131217, LPS-induced in vitro model by AF131217.1 group; LPS+AF131217+miR-128-3p, LPS-induced in vitro model by AF131217.1 and miR-128-3p group. ## P<0.01 compared with LPS+Negative group; ** P<0.01 compared with LPS+ AF131217.1 group. GAPDH=glyceraldehyde- 3-phosphate dehydrogenase; LPS=lipopolysaccharide