Key result
Eight weeks of high-intensity interval training, alone or combined with crocin consumption, significantly reduced doxorubicin-induced elevations in serum LDH and CK-MB levels in male rats.
Why the study?
Doxorubicin is an effective cancer therapy but causes cytotoxicity in healthy tissues, particularly cardiac tissue.
Does high intensity interval training and crocin consumption reduce serum markers of doxorubicin-induced cardiotoxicity in male Wistar rats?
Does high intensity interval training and crocin consumption reduce serum markers of doxorubicin-induced cardiotoxicity in male Wistar rats?
High intensity interval training, alone or combined with crocin, mitigates doxorubicin-induced elevations in cardiac injury markers (LDH and CK-MB) in a rat model.
Answers are grounded in this paper and cite their sources.
HIIT with or without crocin attenuates doxorubicin cardiotoxicity markers in rats; leaves open translation to clinical cardioprotection.
Objective: Doxorubicin (Dox) is a very effective drug for the treatment of a wide range of cancers. However, in healthy tissues, especially cardiac tissue, it can lead to cytotoxicity. This study aims to determine the effect of 8 weeks of high intensity interval training (HIIT) and crocin consumption on serum markers of Dox-induced cardiotoxicity in male rats Methods: In this study, 32 male Wistar rats were randomly divided into four groups: healthy control, doxorubicin, doxorubicin + HIIT, and doxorubicin + crocin + HIIT. The HIIT program was performed for 8 weeks, five days a week, with two 2-min intervals and 80% intensity in the first week and eight 2-minute intervals and 90% intensity of maximum speed in the final weeks. The last group received 10 mg/kg body weight of crocin by gavage for 8 weeks. Dox was injected subcutaneously seven times at a dose of 2 mg/kg. Forty-eight hours after the last training session and after anesthesia, blood samples were taken directly from the left ventricle; after separating serum from plasma, the levels of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) were measured. Data were analyzed using independent t-test and one-way analysis of variance. The significance level was set at 0.05. Results: The Dox injection led to a significant increase in LDH and CK-MB levels in rats compared to the healthy control group (P=0.001). However, 8 weeks of HIIT significantly reduced the serum levels of LDH (P=0.001) and CK-MB (P=0.017) compared to the Dox group. In addition, 8 weeks of HIIT plus crocin consumption significantly reduced the LDH (P=0.001) and CK-MB (P=0.001) levels compared to the Dox group. There was no significant difference between the effects of HIIT alone and HIIT plus crocin consumption on the serum levels of LDH (P=0.087) and CK-MB (P=0.877) in rats received doxorubicin. Conclusion: It seems that HIIT alone and in combination with crocin consumption can reduce the changes in serum levels of LDH and CK-MB induced by doxorubicin.
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Qaracheh et al. (2022) studied Doxorubicin-induced cardiotoxicity (n=32). High intensity interval training (HIIT) and crocin vs. Doxorubicin alone was evaluated on Serum levels of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) (p=0.001). Eight weeks of high-intensity interval training, alone or combined with crocin consumption, significantly reduced doxorubicin-induced elevations in serum LDH and CK-MB levels in male rats.
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