Key result
Serine supplementation significantly alleviated doxorubicin-induced oxidative damage in skeletal muscle and improved exercise capacity in mice.
Why the study?
It remained unknown whether serine supplementation can protect mice against oxidative stress-induced muscle weakness, prompting this test of whether it alleviates doxorubicin-induced oxidative damage in skeletal muscle.
Does serine supplementation prevent doxorubicin-induced oxidative damage in the skeletal muscle of mice?
Does serine supplementation prevent doxorubicin-induced oxidative damage in the skeletal muscle of mice?
Serine supplementation may serve as a nutritional strategy to alleviate doxorubicin-induced oxidative damage and skeletal muscle weakness.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for serine in doxorubicin myoprotection; clinical translation untested and should not change practice.
Muscle weakness affects physical activity and quality of life of patients. Serine, a nutritionally non-essential amino acid has been reported to enhance protein synthesis and implicate in biosynthesis of multiple bioactive molecules. It remains unknown whether it can protect mice against oxidative stress-induced muscles weakness. This study was conducted to test the hypothesis that serine administration alleviates doxorubicin-induced oxidative damage in skeletal muscle of mice. Mice pre-treated with or without serine were intraperitoneally injected with either doxorubicin or equal volume of saline. Reactive oxygen species (ROS) accumulation, activity of antioxidant enzymes, oxidation product of protein, DNA, and lipid, activity of mitochondrial complex, and protein level of nuclear-factor-erythroid-2-related factor 2 (NRF2)/constitutive-androstane-receptor (CAR) signaling in skeletal muscle of mice were determined. Compared with the control, doxorubicin exposure led to oxidative damage as shown by increased ROS accumulation, decreased activity of antioxidant enzymes, and enhanced oxidative product of protein, DNA, and lipid in the skeletal muscle of mice. These effects of doxorubicin were associated with increased activity of complex I and reduced glutathione. Interestingly, doxorubicin-induced oxidative damage was alleviated by serine administration. Further study showed that the beneficial effect of serine was associated with enhanced NRF2/CAR signaling. Our result showed that serine attenuated doxorubicin-induced muscle weakness in mice. Serine supplementation might be a nutritional strategy to improve the function of skeletal muscle in patients exposed to doxorubicin.
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Chen et al. (2021) studied Doxorubicin-induced oxidative damage in skeletal muscle (n=32). Serine vs. Doxorubicin alone or saline control was evaluated on Oxidative damage markers (ROS, MDA, 8-OHdG) and exercise capacity (p=<0.05). Serine supplementation significantly alleviated doxorubicin-induced oxidative damage in skeletal muscle and improved exercise capacity in mice.
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