The amino-acid requirements of Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh. were studied in a medium containing vitamins, glucose, and mineral salts. Seven amino acids, asparagine, methionine, leucine, histidine, alanine, arginine, and proline, were found to be required to obtain growth equivalent to that obtained with casein hydrolyzate, though asparagine and methionine are of major nutritional importance: no absolute requirement for a specific amino acid could be demonstrated. Tryptophane, tyrosine, phenylalanine, cystine, and cysteine were found to be inhibitory.
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R. A. Lachance (1962) studied this question.
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