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A low‐angle light‐scattering instrument has been used to measure molecular weights of native calf‐thymus and T7 DNA. Molecular weights obtained by extrapolation of angular data to 0° from measurements above 30° are less than molecular weights from extrapolation of data taken at low angles (below 30°). The low‐angle molecular weights determined for calf‐thymus DNA and for T7 DNA agree well with estimates of weight‐average molecular weight obtained with other techniques. The low‐angle light‐scattering molecular weight for calf‐thymus DNA is higher than previously reported values by light scattering at angles above 30°. A concentration dependence in the scattering from DNA solutions is also observed.
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Harpst et al. (1968) studied this question.
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