Determination of free and total gossypol by high performance liquid chromatography

Existing HPLC methods determine only pure gossypol whereas the official AOCS method determines both gossypol and other physiologically active gossypol‐like compounds that react with 3‐amino‐1‐propanol and aniline. The feed industry uses the official AOCS method, which is complex and produces results that do not correlate well among laboratories. HPLC methods were developed, using 3‐amino‐1‐propanol as a complexing agent, for the quantitative determination of free and total gossypol in cottonseed meal, oil, and ethanolic miscella. These methods are simple, sensitive, and provide reproducible results. In addition the use of toxic aniline is eliminated.