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February 1, 2019The Indian Journal of Medical ResearchOpen Access

Phenotypic & genotypic profile of antimicrobial resistance in Pseudomonas species in hospitalized patients

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Authors

VBVijeta BajpaiAGAishwarya GovindaswamySKSurbhi Khurana

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Overview

Observational study reveals high phenotypic and genotypic beta-lactamase resistance in clinical Pseudomonas isolates, indicating a critical need for combined surveillance strategies.

Key Points

  • Characterize the phenotypic and genotypic antimicrobial resistance patterns of Pseudomonas species isolated from hospitalized patients.
  • Analyzed 126 consecutive, non-duplicate clinical Pseudomonas isolates collected over a two-year period from various specimen types.
  • Identified isolates and evaluated antimicrobial susceptibility alongside phenotypic screening for ESBLs, AmpC, and MBLs via automated culture, disc-diffusion, and E-test methods following CLSI guidelines.
  • Performed polymerase chain reaction assays to identify beta-lactamase-encoding genes, including blaTEM, blaSHV, blaNDM-1, blaVIM, and blaOXA-1.
  • Pseudomonas aeruginosa comprised 96.1% (n=121) of isolates, with highest resistance rates observed against ticarcillin-clavulanic acid (89.7%, n=113), meropenem (72.5%, n=92), and ceftazidime (72.3%, n=91).
  • Phenotypic assays detected ESBL production in 96.4% (n=109), carbapenemase production in 94.6% (n=105), and AmpC production in 50.8% (n=64) of isolates.
  • blaTEM was the most prevalent ESBL gene (57.1%, n=72), while blaNDM-1 was the most common carbapenemase gene (46.06%, n=41), followed by blaVIM and blaOXA-1.

Cite This Study

Bajpai et al. (2019) studied this question.

synapsesocial.com/papers/6a98ca81d71845e702aa8657https://doi.org/10.4103/ijmr.ijmr_1_18
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