Key result
CD31 deficiency in mice resulted in almost identical kinetics of thrombus formation in venules (vessel occlusion: 5.03 vs 5.05 min) and arterioles compared with wildtype animals.
Why the study?
Does CD31 deficiency affect blood cell thrombus formation upon endothelial cell injury in mice?
Does CD31 deficiency affect blood cell thrombus formation upon endothelial cell injury in mice?
This in vivo study demonstrates that CD31 (PECAM-1) is not critically involved in blood cell thrombus formation following endothelial cell injury.
CD31 is dispensable for thrombus formation after endothelial injury in mice; challenges its proposed role and leaves open human relevance.
A murine model of endothelial cell injury-based vascular thrombosis was used to test the role of platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) in blood cell aggregate formation and vessel occlusion in vivo. Photochemically-induced thrombus formation was analyzed in detail using intravital fluorescence microscopy of individual microvessels in cremaster muscle preparations of CD31-deficient and wildtype mice. In venules, epi-illumination induced rapid thrombus formation with first platelet deposition after 0.56 +/- 0.11 min and complete vessel occlusion within 5.05 +/- 0.45 min. In arterioles, thrombus formation was markedly delayed with first platelet deposition after 3.03 +/- 0.47 min and complete vessel occlusion within 10.04 +/- 1.26 min. Kinetics of thrombus formation in both venules (first platelet deposition: 0.52 +/- 0.1 min; vessel occlusion: 5.03 +/- 0.52 min) and arterioles (first platelet deposition: 3.06 +/- 0.68 min; vessel occlusion: 10.02 +/- 1.38 min) of CD31-deficient mice was found almost identical compared with that in wildtype animals. Tail bleeding time was 233 +/- 24 s in wildtype and 243 +/- 32 s in CD31-deficient mice. Moreover, CD31-deficient and wildtype mice revealed comparable interaction of leukocytes to endothelium. This study shows for the first time in vivo that CD31 is not critically involved in blood cell thrombus formation upon endothelial cell injury.
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Schmits et al. (2001) studied Vascular thrombosis. CD31 deficiency vs. Wildtype mice was evaluated on Kinetics of thrombus formation (first platelet deposition and complete vessel occlusion). CD31 deficiency in mice resulted in almost identical kinetics of thrombus formation in venules (vessel occlusion: 5.03 vs 5.05 min) and arterioles compared with wildtype animals.
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