DecreasedTRPM7inhibits activities and induces apoptosis of bladder cancer cells via ERK1/2 pathway

// Rui Cao 1, * , Zhe Meng 1, * , Tongzu Liu 1 , Gang Wang 1 , Guofeng Qian 2 , Tingting Cao 3 , Xinyuan Guan 3 , Hancai Dan 4 , Yu Xiao 1, 5 , Xinghuan Wang 1 1 Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China 2 Department of Endocrinology, The First Affiliated Hospital of Zhejiang University, Hangzhou, China 3 Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong 4 Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, MD, USA 5 Center for Medical Science Research, Zhongnan Hospital of Wuhan University, Wuhan, China * These authors contributed equally to this work Correspondence to: Yu Xiao, email: yu.xiao@whu.edu.cn Xinghuan Wang, email: wangxinghuan@whu.edu.cn Keywords: TRPM7, bladder cancer, apoptosis, MAPK, AKT Received: March 29, 2016 Accepted: September 13, 2016 Published: September 20, 2016 ABSTRACT Transient receptor potential melastatin 7 (TRPM7) functions as a Mg 2+ /Ca 2+ -permeable channel fused with a kinase domain and regulates various physical processes and diseases. However, its effects on pathogenesis of human bladder cancer (BCa) has not been clarified yet. Our microarray analysis has suggested that calcium signaling pathway is connected with bladder cancer via MAPK pathway. Therefore, we aim to investigate the mechanism of TRPM7 in BCa tumorigenesis by using BCa tissues compared with normal bladder epithelium tissues, as well as using distinct BCa cell lines (EJ, 5637 and T24). We observed increased TRPM7 expression and dysregulation of proteins involved in Epithelial-Mesenchymal Transition (EMT) in BCa tissues. Moreover, knockdown of TRPM7 in BCa cells reversed the EMT status, accompanied by increase of reactive oxygen species (ROS). Furthermore, TRPM7 deficiency could inhibit BCa cell proliferation, migration and invasion, as well as induce p-ERK1/2 and suppress PI3K/AKT at the protein level. Downregulation of TRPM7 promoted cell cycle arrest at G0/G1 phase and apoptosis in vitro , which could be recovered by pre-treatment with U0126 to deactivate ERK1/2, suggesting a close correlation between TRPM7 and the MAPK signaling pathway. Furthermore, a NOD/SCID mouse model transplanted using the BCa cells was established, revealing delayed tumor growth by reduced protein activity and mRNA transcription of TRPM7 in vivo . Our results suggested TRPM7 might be essential for BCa tumorigenesis by interfering BCa cell proliferation, motility and apoptosis.