BACKGROUND: Saccharomyces cerevisiae is one of the main microorganisms that can produce S‐adenosylmethionine (SAM) from L‐methionine and ATP with high productivity. To satisfy the ATP requirement for SAM synthesis, sufficient oxygen should be supplied to the medium to improve aerobic metabolism in S. cerevisiae. In this study, n‐hexadecane used as oxygen vector for enhancement of SAM production by this yeast was investigated. RESULTS: N‐hexadecane was most favorable for cell growth and SAM synthesis in S. cerevisiae when added at the time of inoculation. It could increase glucose consumption, reduce ethanol accumulation, and ultimately improve biomass and SAM productivity in a fermentation process. In a bioreactor, the highest yield of SAM (2.27 g L−1) was achieved in the presence of 4% (v/v) n‐hexadecane after 24 h of inoculation, which was 23.37% higher than the control (1.84 g L−1). CONCLUSION: The addition of n‐hexadecane to cultures of S. cerevisiae significantly enhanced SAM production without increasing energy consumption, and has the potential for use in large‐scale fermentation processes to increase oxygen supply.
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Li et al. (2012) studied this question.