Commercial samples of nicotinamide adenine dinucleotide (NAD) contain various amounts of one or more lactic dehydrogenase (LDH) inhibitors but not the inhibitor described in the reduced coenzyme, NADH. The kinetic assay for LDH, in which a high concentration of NAD is used, was more seriously influenced by the source of NAD than a tetrazolium-coupled colorimetric procedure that requires much less coenzyme.
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Babson et al. (1970) studied this question.