SUMMARY Mass changes were used to monitor water gain or loss during fixation of plant tissue. Mannitol was used as reference osmoticum. Glutaraldehyde in Na‐Na‐phosphate, Na‐cacodylate or PIPES buffer exerts an osmotic effect on plant tissue. This effect is in addition to the osmotic effect of the buffer. The effective osmolarity of the fixative depends on the glutaraldehyde concentration, the buffer type, the buffer concentration and the tonicity of the tissue. Relationships among these parameters are non‐linear. Graphs are given to find the effective osmotic pressure of a buffered or unbuffered fixative containing 2% glutaraldehyde, for use with plant storage tissue with tonicity between 350 and 850 mOsm.
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Coetzee et al. (1986) studied this question.
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