Dual system for proteins: The site-specific two-color labeling of a Rab GTPase was achieved in a one-pot procedure by combination of chemoselective native chemical ligation and oxime ligation. This strategy could be a general, facile, and efficient method for specific multiple modifications of a given protein. The Rab GTPase biosensor was demonstrated for protein folding and protein–protein interaction studies.
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Yi et al. (2011) studied this question.
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