Genetic Identification of Lophius budegassa and L. piscatorius by PCR‐RFLP Analysis of a Mitochondrial tRNA Glu /Cytochrome b Segment

Identification of Lophius budegassa (black‐bellied angler) and L. piscatorius (angler) (Lophiiformes) was carried out on the amplification of a 486 bp tRNA Glu /cytochrome b segment using the polymerase chain reaction (PCR). Direct DNA sequencing of 6 PCR products was carried out. Six restriction endonucleases ( Alu I, Cfo I, Hae III, Hin fI, Mae , and Scr FI) with different species‐specific restriction fragment length polymorphism (RFLP) were selected. Digestions of PCR products from 30 individuals showed no intraspecific polymorphism. Double digestions ( Cfo I and Hin fI, and Hae III and Scr FI) were simpler and more rapid than single digestions. This technique is suitable for distinguishing tails of both Lophius species.