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Aprotocol was developed for molecular characterization of debranched starch amylopectin using Matrix-assisted laser desorption/ionizationmass spectrometry (MALDI-MS). Starch from waxy corn was used in the protocol development. Various factors influencing the protocol were investigated and discussed. Using the developed protocols, starch amylopectin from barley grains (waxy-type Candle and regular-type Phoenix) were also characterized. The study suggested that MALDI-MS is suitable to determine the molecular size profile of debranched starch (maltooligo- and polysaccharides) faster and with greater accuracy than any present analytical methodology.
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Wang et al. (1999) studied this question.