Selective Immobilization of Proteins onto Solid Supports through Split‐Intein‐Mediated Protein Trans‐Splicing

Without a trace: The immobilization of biologically active proteins onto a solid support through split-intein-mediated protein trans-splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates.