Key result
A newly developed multiplex real-time PCR assay successfully defined the viral etiology in 33 out of 50 tested samples of acute respiratory infections.
Why the study?
Does a newly developed multiplex RT-PCR assay accurately identify viral agents causing acute respiratory infections in human samples?
Does a newly developed multiplex RT-PCR assay accurately identify viral agents causing acute respiratory infections in human samples?
The developed multiplex RT-PCR assay demonstrates high sensitivity and specificity for detecting key respiratory viruses, successfully identifying the etiology in 66% of tested clinical samples.
Should not yet alter clinical viral testing; leaves open utility pending larger prospective validation.
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 10 3 genome equivalents per milliliter. Diagnostic sensitivity for flu virus type A and B, and also subtypes H1 (seasonal H1N1, pandemic variant of H1N1 of year 2009), H3, H5 was 1 × 10 3 –10 4 viral particles per milliliter. The method developed has high specificity and does not have positive signal in experiments with DNA/cDNA of human beings and viral DNA. We have studied 50 samples using the developed set. Etiology was defined in 33 samples.
No takes yet. Share an insight, caveat, or question.
Sergeeva et al. (2013) studied Acute respiratory infections (n=50). Multiplex real-time PCR assay was evaluated on Definition of viral etiology in samples. A newly developed multiplex real-time PCR assay successfully defined the viral etiology in 33 out of 50 tested samples of acute respiratory infections.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: