The interference of various concentrations of soluble protein and organic acids with chloride (Cl‐) determinations using a specific ion electrode was evaluated. The organic acids, malic and citric, provided 4 to 5 times the Cl‐ interference as did soluble protein at equivalent concentrations. A definite interaction of the two interfering substances existed which also led to erroneous estimations of the actual Cl‐ concentration. A comparison of corn leaves dry ashed in a basic media versus water extracted resulted in 4 to 5 times hi gher Cl‐values in the water extract. The results of this experiment suggest that water extraction of plant tissue for Cl‐ determinations will result in an overestimation of the actual Cl‐value when using the specific ion electrode. Dry ashing the sample in a basic media, then extracting with hot water results in reliable estimates of the Cl‐ concentration.
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Krieg et al. (1977) studied this question.
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