The extraction of DNA is a basic tool for molecular analyses, which is difficult for grapevine due to its content of secondary metabolites. In terms of purity and yield, DNA extraction kits are superior; however, they are also expensive. Here we describe an efficient protocol to re-use the DNeasy 96 Plant Kit from Qiagen GmbH (Hilden, Germany) by preparing homemade buffers and regenerating the commercial 96 column plates. Leaf tissue extractions of different grapevine samples resulted in genomic DNA with a high molecular weight and sufficient purity suitable for molecular studies like restriction assays or various amplification analyses. This high throughput method is especially economical and does not require the use of laboratory robotics for pipetting steps.
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Lemke et al. (2015) studied this question.
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