The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods

Şöhret Güler; Afşin Köker

doi:10.30704/http-www-jivs-net.1893194

August 31, 2026Journal of Istanbul Veterinary SciencesOpen Access

The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods

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Key Points

To assess and compare the impacts of two vitrification methods, Open Pulled Straw (OPS) and Solid Surface Vitrification (SSV), on the in vitro maturation capacity of immature bovine oocytes.
Sampled 450 immature bovine oocytes with at least three compact cumulus cell layers aspirated from abattoir-derived ovaries.
Allocated oocytes into three groups (N=150 per group): Open Pulled Straw vitrification, Solid Surface Vitrification, and a non-vitrified control group.
Cultured oocytes for in vitro maturation post-warming (or without freezing for controls) and evaluated cumulus expansion and polar body extrusion under a stereomicroscope.
Polar body extrusion was observed in 23.19% (32/138) of oocytes in the OPS group and 20.90% (28/134) in the SSV group, showing no statistically significant difference between vitrification techniques (p > 0.05).
Non-vitrified control oocytes achieved a maturation rate of 78.83% (108/137), which was significantly higher than both vitrification groups (p < 0.05).

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Implication

Comparative study demonstrates reduced maturation rates in vitrified bovine oocytes compared to fresh controls, indicating persistent cryodamage across different freezing techniques.

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Güler et al. (2026) studied this question.

synapsesocial.com/papers/6a9935b1636c6408cfa7e32a https://doi.org/10.30704/http-www-jivs-net.1893194

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The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods

Key Points

No abstract yet

Implication

Discussion

Cite This Study

The achievements of in vitro maturation after warming to the cryopreserved bovine oocytes by different vitrification methods

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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