Key result
Virosomes prepared by inserting vesicular stomatitis virus glycoprotein into preformed liposomes exhibited pH-sensitive fusogenic activity dependent on the glycoprotein and lipid composition.
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A technique was developed to make vesicles fusogenic by inserting vesicular stomatitis virus glycoprotein into preformed liposomes, with activity dependent on pH and lipid composition.
Virosomes were prepared by the insertion of vesicular stomatitis virus glycoprotein, a pH-sensitive fusion protein, into preformed liposomes. The fusogenic activity of these virosomes was characterized in cell-free fusion assays using liposomal targets. Fusion was monitored by concentration-dependent changes in the efficiency of resonance energy transfer between N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine and N-(4-nitrobenzo-2-oxa-1,3-diazol)-phosphatidylethanolamine and by electron microscopy. The fusogenic activity was dependent on the presence of vesicular stomatitis virus glycoprotein, was pH-sensitive, and had a pH threshold of activation similar to that of the native virus. The extent of fusion was dependent upon the lipid composition of the vesicles. This technique will allow vesicles prepared by any method to be made fusogenic.
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Hug et al. (1994) studied this question. Insertion of vesicular stomatitis virus glycoprotein into preformed liposomes was evaluated on Fusogenic activity in cell-free fusion assays. Virosomes prepared by inserting vesicular stomatitis virus glycoprotein into preformed liposomes exhibited pH-sensitive fusogenic activity dependent on the glycoprotein and lipid composition.
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