Pairing probes: Recently, we prepared several probes that monitor protein kinase C activities in living cells based on a pleckstrin fragment sandwiched between two fluorescent proteins, GFP2 and EYFP. Herein, we replaced the fluorescent proteins (FPs) with monomeric variants which resulted in nonfunctional probes. This suggested that fluorophore dimerization actively participated in probe performance.
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Jost et al. (2008) studied this question.