Key result
A microtechnique for plaque assay of Poliovirus type I using plastic chambers achieved 80-85% maximum efficiency of adsorption, with clear plaques appearing within 30-34 hours.
A microtechnique using plastic chambers on glass slides provides a reliable, simple, and economical system for poliovirus plaque assays.
May streamline poliovirus lab workflows; leaves open standardization in clinical virology without further validation.
A method for the plaque assay of Poliovirus type I using plastic chambers on glass microscope slides with an agar overlay has been developed. This microtechnique offers a reliable system for plaque assays which is directly comparable with other systems. The maximum efficiency of adsorption was 80–85% and clear and countable plaques appeared within 30–34 hours. The advantages of this system include simplicity, economy of materials, and flexibility. Other applications of the technique include plaque inhibition, plaque size reduction, immunofluorescent plaque assays, and cytological studies.
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Chaudhary et al. (1969) studied Poliovirus type I. Plaque assay using plastic chambers on glass microscope slides with an agar overlay vs. Other systems was evaluated on Maximum efficiency of adsorption. A microtechnique for plaque assay of Poliovirus type I using plastic chambers achieved 80-85% maximum efficiency of adsorption, with clear plaques appearing within 30-34 hours.
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