An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of alpha-1,5-L-arabinan and showed no activity towards p-nitrophenyl-alpha-L-arabinofuranoside and alpha-1,5-L-arabinofuranobiose. Abnx is the first enzyme with this mode of action.
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Sakamoto et al. (2001) studied this question.
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