Key result
The high-throughput pseudovirus neutralization test (HTpPNT) showed high correlation with the conventional test (R2 = 0.84-0.90 for types 1-3) and identified age-dependent differences in neutralizing antibody titers against wild-type versus Sabin strains.
Why the study?
Conventional poliovirus neutralization testing requires live strains, motivating the establishment of a high-throughput pseudovirus neutralization test for large-scale serosurveillance.
Does HTpPNT correlate with cPNT for measuring anti-poliovirus neutralization antibody titres in human serum?
Observational (n=731)
Does HTpPNT correlate with cPNT for measuring anti-poliovirus neutralization antibody titres in human serum?
Effect estimate: R2 = 0.84-0.90
HTpPNT is a highly correlated, safe, and high-throughput alternative to conventional live-virus neutralization tests for large-scale poliovirus serosurveillance.
HTpPNT offers a safer high-throughput alternative for PV serosurveillance; leaves open large-scale validation against conventional testing.
To monitor vulnerability of countries to poliovirus (PV) outbreaks, serosurveillance of anti-PV neutralization antibody is conducted by conventional PV neutralization test (cPNT), which uses live PV strains. We previously developed a pseudovirus PV neutralization test (pPNT) as an alternative to cPNT, which uses PV pseudovirus that expresses luciferase as a reporter in the infection without producing infectious PV. In the present study, we established a high-throughput pPNT (HTpPNT) for a large-scale serosurveillance. The HTpPNT system was evaluated with 600 human serum samples obtained from a broad range of age groups of healthy volunteers (ages of 0–89 years). HTpPNT showed high correlation with cPNT (R2 for anti-type 1, 2, and 3 PV neutralization antibody titres are 0.90, 0.84, and 0.90, respectively). By using HTpPNT, we analyzed relative neutralizing antibody titre of the sera against a type 1 PV wild-type strain (Mahoney strain) to that against the type 1 Sabin strain. As a result, a correlation between the age (≥ 60 years) and the relative neutralizing antibody titre was observed (n = 15–16, P = 0.0000023–0.041), while the types of PV vaccine (i.e., oral PV vaccine and Sabin strain-based IPV) had no effect. HTpPNT would serve as a useful alternative to cPNT in a large-scale serosurveillance.
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Arita et al. (2022) conducted an observational in Healthy volunteers (poliovirus serosurveillance) (n=731). High-throughput pseudovirus neutralization test (HTpPNT) vs. Conventional PV neutralization test (cPNT) was evaluated on Correlation of anti-poliovirus neutralization antibody titres between HTpPNT and cPNT (R2 = 0.84-0.90). The high-throughput pseudovirus neutralization test (HTpPNT) showed high correlation with the conventional test (R2 = 0.84-0.90 for types 1-3) and identified age-dependent differences in neutralizing antibody titers against wild-type versus Sabin strains.
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