After growing Pseudomonas aeruginosa in the presence of 2 ,4-[4-15N]-diaminobutyric acid (Dab) its incorporation into the quinoline chromophore of the pyoverdin produced by this bacterium could be shown by mass and NMR-spectroscopic techniques. In combination with earlier results it can thus be stated that the precursor of the chromophore is a condensation product of ʟ-Dab and ᴅ-Phe or ᴅ-Tyr. A synthesis for [4 -15N ]-Dab is described.
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Böckmann et al. (1997) studied this question.