Summary A controlled system has been designed for the in vitro study of antibody synthesis. To avoid difficulties in interpretation, coprecipitation of radioactive impurities has been controlled by comparison of the radioactivity in a homologous specific precipitate to that in a closely related heterologous specific precipitate made in the same medium. Animals exhibiting the anamnestic response were superior for the in vitro study of antibody synthesis. Synthesis of radioactive antibody was demonstrated in isolated rabbit spleen, lymph node, lung and liver slices. Production of radioactive antibody in each organ studied is in accord with the concentration and distribution of cell types believed to be responsible for antibody synthesis.
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Keston et al. (1956) studied this question.