The gene coding for epidermolytic toxin serotype B (etb) was cloned in a plasmid expression vector in Escherichia coli. Its expression was dependent on the vector plasmid's trc promoter. A polypeptide immunochemically indistinguishable from the purified staphylococcal toxin and with the same molecular weight was predominantly localized in the periplasm of E. coli. The etb gene resides on a 1.7 kb HindIII fragment of the 42 kb plasmid pTC142 present in the parental Staphylococcus aureus strain.
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O’Toole et al. (1986) studied this question.
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