Microarray and proteome array in an atherosclerosis mouse model for identification of biomarkers in whole blood

Figures

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  Figure 1. Histological analysis of the aorta from ApoE+/+ and ApoE-/- mice. (A) The oil red O stain of aorta in ApoE+/+ (left panel) and ApoE-/- (right panel) mice. Arrow indicates atherosclerotic lesions stained by oil red O. Scale bars = 1 mm. (B) Lesion area quantification. (C) Number of stained spots. Data represent mean ± S.E.M. Experiments were performed three times independently. *P < 0.05, ***P < 0.001.

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  Figure 2. qRT-PCR analysis of target genes in blood of ApoE+/+ and ApoE-/- mice. (A) Upregulated and (B) downregulated target genes are shown. GAPDH was used as the housekeeping gene. Data represent mean ± S.E.M. Experiments were performed three times independently. *P < 0.05, **P < 0.01.

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  Figure 3. Protein levels in blood of ApoE+/+ and ApoE-/- mice. (A) Representative proteome array panel. (B) Fold change in spot density of (A). Relative fold change corresponds to the density of spot in ApoE-/- compared with control spot. Density was normalized with density of blanks, negative, and positive controls. Data represent mean ± S.E.M. Experiments were performed three times independently.

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  Figure 4. qRT-PCR analysis of target genes in ApoE+/+ and ApoE-/- mice. The expression of each gene was confirmed using specific primers. GAPDH was used as the housekeeping gene. Data represent mean ± S.E.M. Experiments were performed three times independently. *P < 0.05, **P < 0.01, *** P < 0.001.

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  Figure 5. Protein expression in ApoE+/+ and ApoE-/- mice measured using ELISA. Data represent mean ± S.E.M. Experiments were performed three times independently. *P < 0.05.