Hepatic small extracellular vesicles promote microvascular endothelial hyperpermeability during NAFLD via novel-miRNA-7

Figures

• 

  Fig. 1. Endothelial permeability is enhanced in coronary microvessels of NAFLD mice. Mice were fed a MCD diet for 4 weeks or HFD for 16 weeks to induce NAFLD. A Representative images of liver H&E staining, oil red O staining and B-scan ultrasonographic imaging, n = 6 per group. Scale bar: 200 μm. B Liver triglyceride (TG) content, n = 6 per group. C The ratio of hepatic-renal echo-intensity, n = 6 per group. D, E Plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), n = 6 per group. F Representative images and the summarized data of Evans blue concentration in heart tissues, n = 6 per group. G Representative fluorescent confocal images of ZO-1/2 (green) with vWF (red) and the summarized data of the Manders overlap coefficient of ZO-1/2 over vWF. The area of interest (AOI) is selected for higher magnification, n = 6 per group. Scale bar, 20 μm. Data are expressed as the mean ± SEM. Statistics: Student t-test, **P < 0.01 vs. the MCS group; &&P < 0.01 vs. the ND group

• 

  Fig. 2. NLRP3 inflammasome mediates endothelial hyperpermeability of coronary microvessels in NAFLD mice. The human targets of microvascular hyperpermeability was gathered from the GeneCards database, and the Gene Ontology (GO) analysis was performed to analyze the main function of target genes. NLRP3+/+ and NLRP3−/− mice were fed a MCD diet for 4 weeks or HFD for 16 weeks to induce NAFLD. A, B GO enrichment analysis of the human targets of microvascular hyperpermeability. The size of the circles represents the number of child GO term. The color represents the significance of the enrichment or the category of molecular function. BP: Biological Process, CC: Cellular Component, MF: Molecular Function. C–F Representative fluorescent confocal images of cleaved-caspase-1(CASP1), IL-1β, and ZO-1/2 (green) with vWF (red) and the summarized data of the Manders overlap coefficient. The area of interest (AOI) is selected for higher magnification, n = 6 per group. Scale bar, 20 μm. G, H Representative images and the summarized data of Evans blue concentrations in heart tissues, n = 6 per group. Data are expressed as the mean ± SEM. Statistics: One-way ANOVA, *P < 0.05, **P < 0.01 vs. NLRP3+/+ mice…

• 

  Fig. 3. Circulating NAFLD hepatic sEV induces NLRP3 inflammasome-dependent endothelial hyperpermeability in coronary microvessels. NAFLD or control hepatic sEVs were isolated by differential ultracentrifugation, identified, and administered to naive NLRP3+/+ and NLRP3−/− mice via caudal vein injection. A Representative electron micrograph of sEVs reveals the morphology and size. Scale bar, 200 nm. B Size distribution analysis of sEVs by nanoparticle tracking analysis. C Western blot analyses of sEV markers, including CD63, HSP70, and TSG101. D In vivo optical imaging system-obtained fluorescence imaging of cardiac tissue, n = 6 per group. The pellet derived from the ultracentrifugation of DiR alone was used as a vehicle control. E–H Representative fluorescent confocal images of cleaved-caspase-1(CASP1), IL-1β, and ZO-1/2 (green) with vWF (red) and the summarized data of the Manders overlap coefficient. The area of interest (AOI) is selected for higher magnification, n = 6 per group. Scale bar, 20 μm. I, J Representative images and the summarized data of Evans blue concentrations in heart tissues, n = 6 per group. Data are expressed as the mean ± SEM. Statistics: One-way ANOVA, *P < 0.05,…

• 

  Fig. 4. NAFLD hepatic sEVs induce microvascular endothelial hyperpermeability by activating Cathepsin B-dependent NLRP3 inflammasome axis. A MVECs were pretreated with or without 20 μmol/L dynasore for 40 min, followed by incubation of 120 μg/mL DiI-labeled hepatic sEVs for 8 h. Representative fluorescent confocal images of DiI-labeled hepatic sEVs (red) with DAPI (blue), n = 4 per group. The pellet derived from the ultracentrifugation of DiI alone was used as a vehicle control. Scale bar, 10 μm. B Representative western blot bands and the summarized data determined by densitometric analysis, n = 4 per group. C Representative flow cytometry images of caspase-1 FLICA staining and the summarized data of the positive cells, n = 4 per group. The fold changes were obtained by calculating the ratio of the positive cells of the treated groups to the MCS or ND group. D Representative Western blot bands and the summarized data determined by densitometric analysis, n = 4 per group. E Relative permeability of endothelial monolayer to FITC-dextran, n = 4 per group. F Representative images of acridine orange (AO) and magic red (MR) staining, n = 4 per group. Scale bar, 20 μm. G MVECs were incubated…

• 

  Fig. 5. Novel-miR-7 is a key hepatic sEV cargo that promotes microvascular endothelial hyperpermeability by targeting LAMP1. Hepatic sEV miRNAs were profiled by the small RNA sequencing analysis. MVECs were incubated with 120 μg/mL hepatic sEVs for 24 h (D) or transfected with 50 nmol/L novel-miR-7 mimics or negative control (NC) mimic (E–K). A The heat-map of the ten miRNAs with the most significant (fold change > 10, FDR < 0.05) abundance differences in hepatic sEVs derived from the MCD group versus the MCS group, n = 3 per group. B, C Quantitative PCR analysis of novel-miR-7 expression levels in hepatic sEVs and mouse plasma-derived sEVs, n = 6 per group. D Quantitative PCR analysis of novel-miR-7 expression levels in hepatic sEVs-treated MVECs, n = 4 per group. E Transfection efficiency of 5-FAM labeled novel-miR-7 mimics. Scale bar, 20 μm. F Representative images of acridine orange (AO) and magic red (MR) staining, n = 4 per group. Scale bar, 20 μm. G Representative Western blot bands and the summarized data determined by densitometric analysis, n = 4 per group. H Representative flow cytometry images of caspase-1 FLICA staining and the summarized data of the positive cells, n = 4…

• 

  Fig. 6. Genetic inhibition of novel-miR-7 ameliorates NAFLD hepatic sEV-induced microvascular endothelial hyperpermeability. MVECs were transfected with 100 nmol/L novel-miR-7 inhibitor or negative control (NC) inhibitor and incubated with or without 120 μg/mL NAFLD hepatic sEVs for 24 h. A Representative western blot bands and the summarized data determined by densitometric analysis, n = 4 per group. B Representative images of acridine orange (AO) and magic red (MR) staining, n = 4 per group. Scale bar, 20 μm. C Representative western blot bands, n = 4 per group. D The summarized data of caspase-1, IL-1β and supernate HMGB1 of WB analysis determined by densitometric analysis, n = 4 per group. E The summarized data of ZO-1 and ZO-2 of WB analysis determined by densitometric analysis, n = 4 per group. F Representative flow cytometry images of caspase-1 FLICA staining, n = 4 per group. G The summarized data of the positive cells of FLICA staning, n = 4 per group. The fold changes were obtained by calculating the ratio of the positive cells of the treated groups to the NC inhibitor group. H Relative permeability of the endothelial monolayer to FITC-dextran, n = 4 per group. Data are…

• 

  Fig. 7. Steatotic hepatocyte is a significant source of novel-miR-7-enriched sEV. Human hepatocyte cell line were treated with 100 μmol/L palmitic acid (PA) or vehicle for 18 h. Hepatocyte sEVs in the HepG2 cell culture media were collected by ultracentrifugation. Human microvascular endothelial cell line-1 (HMEC-1) was transfected with 100 nmol/L novel-miR-7 inhibitor or negative control (NC) inhibitor, and incubated with Ctrl-sEVs or PA-sEVs from HepG2 for 24 h. A HepG2, HuH7 and L02 were treated with 100 μmol/L PA or vehicle for 18 h. SEVs were collected from the supernatant and the expression levels of novel-miR-7 were measured by qPCR analysis, n = 4 per group. B Representative western blot bands and the summarized data determined by densitometric analysis, n = 4 per group. C Representative images of acridine orange (AO) and magic red (MR) staining, n = 4 per group. Scale bar, 20 μm. D Representative flow cytometry images of caspase-1 FLICA staining and the summarized data of the positive cells, n = 4 per group. The fold changes were obtained by calculating the ratio of the positive cells of the treated groups to the NC inhibitor group. E Relative permeability of the endothelial…