Statistical Optimization of Culture Media and Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology

Aim: In view of the increase in Saccharomyces cerevisiaemannan content, the culture medium and condition forS.cerevisiaewere optimized in this study . Study Design: The mathematical model was established by the quadratic rotary combination design via Response Surface Methodology to evaluate the effect of culture condition and media on mannan content in the yeast cell wall. Methodology: The influence of culture mediu m ingredients such as carbon source (FOS, in the range of 2 �9 g/100ml) and nitrogen source (mixture of peptone and yeast extract, in the range of 1-5g/100ml), and enzymeactivator ( glycerol, in the range of 1 -4g/100ml) on mannan production were evaluatedusing Response surface methodology. Also The influence of original pH (in the range of 4 -7), inoculumssize(inthe range of 2-4%) and temperature (in the range of 26