It has been found that the complete separation of ascorbic acid and uric acid on a column packed with newly developed rigid-type polyvinyl alcohol gels by injecting an aliquot (10 μ1) of body fluid without any pretreatment such as deproteinization prior to chromato-graphy can be achieved with good reproducibility. The eluate from the column was amperometrically monitored by aid of an electrochemical detector set at +800 mV vs. Ag/AgCl.
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Yoshiura et al. (1986) studied this question.
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