To examine whether or not the 18S.26S ribosomal RNA genes are located on the B chromosomes of rye, we applied conventional and molecular cytological techniques to the B chromosomes of six rye strains, including cultivated, weedy and wild species. The results indicated that the B chromosomes in the rye genome do not generally carry repeats of the 18S.26S rRNA genes detectable at the cytological level.
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Niwa et al. (1992) studied this question.
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