Application of Fe+2-5-PyridylBenzodiazepin-2-Ones to the Automated Determinations of Serum Iron and Iron-Binding Capacity

An automated procedure has been developed for determining serum iron content and iron-binding capacity with a new iron-chelating ligand, a 5-pyridyl-benzodiazepin-2-one derivative. A simplified manifold and flow system, together with improved reagent combinations, offer distinct advantages over existing automated procedures. The results obtained by automated analysis for serum iron and iron-binding capacity agree excellently with those obtained by the corresponding manual procedure.