Light Boundaries and the Coupled Effects of Surface Hydrophobicity and Light on Spore Settlement in the Brown Algahincksia Irregularis(phaeophyceae)1

We examined the effects of light and surface hydrophobicity individually and in tandem onHincksia irregularis(Kützing) Amsler spore settlement.Hincksia irregularisspores were determined to be negatively phototactic by the use of computer‐assisted motion analysis. Spore settlement was significantly influenced by surface hydrophobicity and by light, individually and in tandem. Experiments conducted using culture plates modified to reduce well edge artifacts revealed significantly higher settlement on hydrophobic surfaces and in dark environments when compared with negatively charged surfaces and lighted environments. Experimental light/dark boundaries elicited distinct spore settlement responses, with spores displaying dissimilar settlement patterns on plates with different surface hydrophobicities. The results of this study indicateH. irregularisspores possess the capacity for complex responses to their environment. These complex responses may influence dispersal and aid spores in the detection of suitable settlement locations in marine microenvironments.