Mass Fabrication of Small Cell Spheroids by Using Micro‐patterned Tissue Culture Plate

A newly designed micro-patterned chamber was utilized to fabricate cell spheroids with a constant size (<200 μm) and cell number. By applying cytochalasin D as a chemical to control cell adhesion and aggregation, thousands of aggregated cells were formed in each patterned chamber. Importantly, the formed cell spheroids were collected by a simple pipetting process without using proteinase.